Epigenetics is the study of factors involved in gene transcription changes without a modification in the DNA sequence: these mechanisms change how genes are expressed without altering the genotype.
MicroRNAs (miRNAs) are small non-coding RNAs involved in transcriptional and post-transcriptional regulation of gene expression. The modification of miRNAs expression has been associated with numerous diseases like cancer.
Agilent miRNA microarray platform enables comprehensive miRNA expression profiling with the most up-to-date content from miRBase. Thanks to a flexible approach and with the help of Helixio experts, we can change and update content with multiple formats to design a custom microarray.
It is also possible to study miRNA expression profiling on Illumina sequencing platform.
Methylation is the best-known epigenetic modification: it corresponds to the addition of a methyl group to the 5’ position of the base cytosine and can result in gene silencing. CpG islands are specific GC-rich sequences; they are often un-methylated in mammals and associated with development. Aberrations in DNA methylation are associated with diseases.
Helixio offers epigenetics services based on Agilent and Illumina microarrays solutions and adapts the proposals to the needs and the specifications of your study.
Agilent Technologies provide two types of microarray, CpG and methylation, to understand epigenetics events leading to gene expression regulation. For example, for human CpG array, probes cover 27,800 islands (21Mb). Human Methylation microarray is focused on CpG islands and promoter regions. Illumina provide HumanMethylation450 BeadChip array, powered by the Infinium methylation assay, for methylation measurement at single CpG site level. It includes 99% of RefSeq genes and 95% of CpG islands.
Helixio can design your custom microarray to focus on your research needs by selecting probes from optimized tiling catalog. Multiple formats are available, just ask us.